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Fig. 4 | Clinical Epigenetics

Fig. 4

From: ARID1A facilitates KRAS signaling-regulated enhancer activity in an AP1-dependent manner in colorectal cancer cells

Fig. 4

ARID1A is localized to AP1-occupied enhancers. ARID1A co-localizes with the BAF complex subunits SMARCA4 and SMARCC1 as well as the active mark H3K27ac. The heatmaps represent all ARID1A binding sites ordered in descending order of ARID1A occupancy (a). ARID1A localizes mainly to regions that are distal to transcription start sites (TSS) with the maximum number of peaks between 5 and 500 kb of TSSs (b). ARID1A-bound enhancers were defined based on overlap of ARID1A with H3K27ac and ATAC-seq. From these regions, any annotated TSSs were subtracted and 3061 ARID1A-bound enhancers were identified (c). Motif analysis on ARID1A-bound enhancers shows enrichment for the Jun-AP1(bZIP) motif (d). ReMap analysis shows that several AP1 transcription factors such as FOSL1, FOSL2, FOS, and JUND are enriched on ARID1A-occupied regions identified in HCT116 cells (e). Co-localization of JUND and FOSL1 at ARID1A-bound enhancers in HCT116 cells is shown. The heatmaps represent the 3061 ARID1A-bound enhancers in descending order of ARID1A occupancy (f)

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