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Table 3 Robust multivariable regression analysis of cg05575921 methylation association with smoking

From: Smoking-associated AHRR demethylation in cord blood DNA: impact of CD235a+ nucleated red blood cells

Model

Model co-variates

Smoking parameter estimate

Smoking parameter estimate, p value*

Model adjusted r2

Model 1. Adjusted for 6 cell types (estimated without nRBCest)

cg05575921 = smoking status + gestational age + infant sex + race + CD8 T cells + CD4 T cells+ natural killer cells + B cells + monocytes + granulocytes

− 0.1284

2.11E−20

0.60

Model 2. Adjusted for 6 cell types (estimated without nRBCest) and log10nRBCabs

cg05575921 = smoking status + gestational age + infant sex + race +CD8 T cells + CD4 T cells+ natural killer cells + B cells + monocytes + granulocytes + log10nRBCsabs

− 0.1388

1.69E−49

0.66

Model 3. Adjusted for 7 cell types (including nRBCest)

cg05575921 = smoking status + gestational age + infant sex + race + CD8 T cells + CD4 T cells+ natural killer cells + B cells + monocytes + granulocytes + nRBCest

− 0.1251

1.69E−33

0.61

  1. Models 1 and 2 used cell type percentages calculated with the 6 cell-type de Goede deconvolution model [11]
  2. *The statistical significance cutoff following adjustment for multiple comparisons is p < 0.0125