Fig. 3
From: Identification of dynamic glucocorticoid-induced methylation changes at the FKBP5 locus
Dexamethasone (DEX)-induced transient changes in hormonal, FKBP5 mRNA and methylation levels in an independent study. a Serum adrenocorticotropin (ACTH) and cortisol (CORT) levels as well as whole blood FKBP5 mRNA levels after an oral dose of DEX in 89 subjects are shown. Peripheral blood was drawn just before administration of DEX (time = 0) as well as 3 and 20–24 h thereafter. The mean and SEM are presented for each time point. As observed in study 1, significant effect across time for ACTH (p value < 2.2e−16) and CORT (p value < 2.2e−16) levels as well as FKBP5 mRNA levels at 3 h (p value < 2.2e−16) are observed. b Boxplots of CpG methylation levels for amplicons located in the intronic enhancers (top), proximal enhancer (bottom left), and topologically associating domain (TAD) boundaries (bottom right) showing replication of DEX-induced methylation changes. X axes indicate the coordinate of each site or region represented. Shaded boxes indicate sites where significant DEX effects were observed at FDR ≤ 0.05 and absolute delta methylation (Ti-baseline) ≥ 1% in at least one time point