Fig. 2

Dexamethasone (DEX)-induced DNA methylation changes (DMCs) within glucocorticoid receptor (GR) and CTCF consensus binding motifs. a Blood methylation levels at baseline across CpGs located within ± 50 bp of GR (left) and CTCF (right) consensus binding motifs. Line plots represent mean and SEM for each CpGs within the region. b Histogram representing counts of CpGs located within 50 bp of motifs (n = 16 for GR (left) and n = 9 for CTCF (right)) where DEX-induced DMC counts are shown in black. c Fold change FKBP5 mRNA expression and ∆ methylation (%) in DMCs located within motifs are shown. The left panel illustrates average ∆ methylation (%) of all DMCs located within GR motifs (n = 4) where each of them is significant after 3 h and 6 h of DEX treatment. The right panel shows ∆ methylation (%) for the 2 DMCs located within CTCF motifs where 1 shows DEX effect after 3 h and 6 h (located at + 24 bp) and the other one only after 23 h (located at + 34 bp). Plots represent mean and SEM