From: Neuronal methylome reveals CREB-associated neuro-axonal impairment in multiple sclerosis
 | Pilot samples | Cohort 1 | Cohort 2 | Cohort IF | ||||
---|---|---|---|---|---|---|---|---|
Group | Case | Control | Case | Control | Case | Control | Case | Control |
N individualsa | 1 | 1 | 5 | 5 | 10 | 7 | 7 | 8 |
Sex ratio (F/M) | 1:0 | 0:1 | 4:1 | 1:4 | 7:3 | 3:4 | 6:1 | 3:5 |
Age (mean ± SD) | 42 | 92 | 45.4 ± 4.5 | 68.4 ± 21.6 | 60.3 ± 12.4 | 78.5 ± 7.9 | 63.6 ± 12.8 | 78.2 ± 5.1 |
PMI (mean ± SD) | 11 | 13 | 17 ± 8.7 | 21 ± 7.4 | 17 ± 9.3 | 11 ± 11.4 | 15 ± 8.0 | 23 ± 3.7 |
N brain samples | 4 | 1 | 7 | 5 | 10 | 7 | 7 | 8 |
Type lesion (N) | AL (1) |  | AL (2) |  | – |  | – |  |
CAL (1) | – | – | – |  | ||||
CL (1) | CL (2) | CL (2) | – |  | ||||
NAWM (1) | NAWM (3) | NAWM (8) | NAWM (7) | Â | ||||
Sample type | Bulk tissue and neurons | Neurons | Neurons | Meurons | ||||
Location | GM, WM, mixed | WM | WM | WM and GM | ||||
Modification | DNA methylation | DNA methylation | DNA methylation | Protein phosphorylation | ||||
Analysis | Bulk BS (mixed 5mC/5hmC) | Bulk BS (mixed 5mC/5hmC) | BS and oxBS (5mC, 5hmC) | IF: P-CREB |