Fig. 6

Identification of XIAP as a direct target of miR-340-5p. a Location, sequence, and predicted binding of miR-340-5p seed region to seed region binding site 2 (SRBS 2) on 3′-UTR of XIAP mRNA, and the design of mutant sequence. b Direct sequencing confirmed the sequences of both wild-type (5′-CTTTATAA-3′) and mutant (5′-CGGGGGAA-3′) in the corresponding luciferase reporter vector. c By qRT-PCR, miR-340-5p was overexpressed when co-transfection with luciferase reporter vector containing wild-type or mutant SRBS 2. d Normalized luciferase activity of luciferase reporter vector carrying wild-type or mutant SRBS 2, in the presence of miR-340-5p mimics or scramble control. Error bars represent the standard deviation from three independent transfections