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Fig. 5 | Clinical Epigenetics

Fig. 5

From: UHRF1 depletion and HDAC inhibition reactivate epigenetically silenced genes in colorectal cancer cells

Fig. 5

Additional HDAC inhibition in UHRF1-depleted CRC cells does not induce further DNA demethylation but does induce histone acetylation of epigenetically silenced genes. a Bisulfite pyrosequencing analysis of epigenetically silenced genes in DLD1 and RKO cells. Cells were transfected with the indicated siRNAs and incubated for 48 h (DLD1) or 72 h (RKO), after which they were treated with or without TSA for 24 h. Cells treated with 5-aza-dC are shown as positive controls for demethylation. b Bisulfite sequencing analysis of the SFRP1 CpG island in DLD1 cells with the indicated siRNAs and treatment. Open and filled circles depict unmethylated and methylated CpG sites, respectively. Locations relative to the transcription start site are shown below. c Density plots of the Infinium BeadChip assay results in DLD1 and RKO cells with the indicated siRNAs and treatment

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