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Fig. 3 | Clinical Epigenetics

Fig. 3

From: UHRF1 depletion and HDAC inhibition reactivate epigenetically silenced genes in colorectal cancer cells

Fig. 3

Demethylation induced by UHRF1 depletion leads to limited gene re-expression in CRC cells. a Results of BeadChip assays showing demethylation of MLH1 induced by UHRF1 knockdown in RKO cells. Shown are β values of BeadChip probes located in the promoter region of MLH1. Locations of the CpG island and the first exon of MLH1 are indicated on the top. b Bisulfite sequencing analysis of the MLH1 CpG island in RKO cells transfected with the indicated siRNAs. c Bisulfite pyrosequencing analysis of MLH1 in RKO cells transfected with the indicated siRNAs or those treated with 5-aza-dC. d RT-PCR of MLH1 in RKO cells transfected with the indicated siRNAs or those treated with 5-aza-dC

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