ZBTB12 DNA methylation is associated with coagulation- and inflammation-related blood cell parameters: findings from the Moli-family cohort

Table 3 Association between ZBTB12 methylation factors and blood cell parameters

	N	Mean	SD	Associations between methylation factors and phenotypes
				N	Factor 1			Factor 2
				N	Beta	SE	p	Beta	SE	p
Functions
Coagulation time (sec.)	417	395.51	77.66	313	− 0.007	0.052	0.891	0.051	0.052	0.333
TNFɑ-stim. coagulation time (sec.)	417	350.90	72.89	313	− 0.021	0.056	0.709	0.160	0.056	0.0047*
Delta coag. time (basal-TNF) (sec.)	417	44.60	56.53	313	− 0.034	0.052	0.510	0.145	0.052	0.0053*
Platelet-monocyte aggr. (%)	450	7.81	9.06	337	0.107	0.045	0.019	0.005	0.046	0.912
Platelet-PMN aggr. (%)	449	4.43	4.97	336	0.032	0.049	0.509	− 0.016	0.050	0.743
Blood cell count
White blood cells (10⁹/L)	458	6.38	1.48	318	− 0.161	0.054	0.0032*	− 0.036	0.055	0.509
Lymphocyte (10⁹/L)	458	2.00	0.58	318	− 0.065	0.056	0.254	− 0.063	0.057	0.271
Monocytes (10⁹/L)	458	0.42	0.17	318	− 0.076	0.052	0.147	0.023	0.053	0.661
Granulocytes (10⁹/L)	458	3.96	1.17	318	− 0.158	0.056	0.0048*	− 0.032	0.056	0.567
Platelets (10⁹/L)	458	253.82	61.84	318	− 0.050	0.052	0.335	− 0.044	0.053	0.407
Red blood cells (10⁹/L)	458	4.91	0.51	318	− 0.046	0.049	0.352	0.003	0.049	0.945

Model adjusted by age, gender, and smoking as fixed effects and family stratification as a random effect; additional covariates were added to the model and were associated to both methylation factors and phenotypes (for blood cell counts, alcohol and obesity). Standardized values of phenotypes and methylation are reported (beta values reported as standard deviation units). Delta coag. time is obtained by the difference between unstimulated and TNFɑ-stimulated coagulation time. Significant p values are shown in italics
*pFDR significant

ISSN: 1868-7083