Fig. 9
From: Identification of a novel quinoline-based DNA demethylating compound highly potent in cancer cells
Effects of MC3353 on osteoblastic differentiation in osteosarcoma cells. a qRT-PCR expression of representative osteoblast differentiation markers in Saos-2 osteosarcoma cells treated with MC3353 at indicated concentrations under osteoblastic conditions. COL1A2, ALP, and OCN are displayed to sign early, intermediate, and late stages of the differentiation process, respectively. The relative mRNA expression levels of target genes (RQ) were calibrated on untreated cells (CTR) at each time point. Data are the mean of at least two independent experiments; SD, standard deviation. b ARS staining of a mineralized matrix over 7–21 days of Saos-2 cells treatment with MC3353 or DAC at indicated concentrations in differentiation medium. Scale bar indicates 200 μm