Skip to main content

Advertisement

Table 1 Clinical characteristics of study participants undergoing controlled ovarian hyperstimulation (COH) assessed before and after oocyte retrieval

From: DNA methylome profiling of granulosa cells reveals altered methylation in genes regulating vital ovarian functions in polycystic ovary syndrome

Parameters assessed Control (n = 20) median (IQR) PCOS (n = 20) median (IQR) P value
Age in years 30.0 (27.0–31.0) 31.5 (28.0–33.0) 0.124
BMI (kg m−2) 23.03 (20.97–25.06) 24.65 (22.24–27.76) 0.173
#Basal FSH (μU/mL) 7.64 (5.52–9.39) 5.71 (4.39–6.61) 0.037*
#Basal LH (μU/mL) 6.19 (3.0–7.66) 9.13 (5.58–12.24) 0.02*
#LH:FSH 0.75 (0.55–1.04) 1.74 (1.15–2.04) 0.0001***
#Prolactin (ng/mL) 14.2 (12.0–18.27) 14.48 (11.72–24.3) 0.43
#TSH (mIU/mL) 1.8 (1.14–2.75) 2.36 (1.55–4.08) 0.0577
#AMH (ng/mL) 3.4 (1.94–7.86) 7.99 (5.21–9.74) 0.0094**
Regular cycle 20 (100%) 3 (15%)  
Oligomenorrhea 0 (0%) 15 (80%) <0.0001***
Secondary amenorrhea 0 (0%) 2 (5%)  
rFSH (IU) 1600 (1528–2313) 1706 (1350–2025) 0.788
E2 (ng/mL) before hCG administration 1.49 (1.247–2.24) 2.03 (1.22–2.47) 0.367
E2 (ng/mL) on hCG administration day 1.71 (1.436–2.32) 2.56 (1.82–3.72) 0.038*
P4 (ng/mL) before hCG administration 0.3 (0.2–0.55) 0.25 (0.17–0.525) 0.522
P4 (ng/mL) on hCG administration day 2.65 (2.07–4.05) 4.6 (1.95–6.32) 0.200
Total follicles (n) 15.0 (12.0–20.5) 18.5 (15.0–29.25) 0.099
Total oocytes (n) 13.5 (10.0–17.25) 16.5 (14.5–26.75) 0.059
MII oocytes (n) 10.5 (8.0–13.5) 15.0 (9.0–19.75) 0.057
MII oocytes (%) 82.84 (70.24–90.71) 86.85 (74.2–95.31) 0.366
Total fertilized oocytes (n) 6.0 (4.5–9.25) 6.5 (3.75–14.5) 0.464
Rate of MII oocyte fertilization (ROF) 65.63 (51.92–88.13) 52.27 (34.8–67.) 0.039*
$E2 (ng/mL) Serum 0.2 (0.15–0.6) 0.43 (0.15–0.76) 0.418
$E2 (ng/mL) FF 374 (178.6–541.7) 280.3 (125–819) 0.586
$P4 (ng/mL) Serum 1 (3–9) 3.9 (0.3–7.) 0.903
$P4 (ng/mL) FF 8170 (4760–15,880) 6000 (3280–10,140) 0.325
$TT (ng/dL) Serum 126 (89–178.5) 172.6 (140–311.3) 0.020*
$TT (ng/dL) FF 300 (140.2–372.6) 405.8 (222.6–639.3) 0.030*
$SHBG (nmol/L) Serum 130 (103.0–150.0) 95 (79–134.5) 0.041*
$SHBG (nmol/L) FF 158 (127.8–205.5) 127.7 (103.3–148.8) 0.024*
$Free T (pmol/L) Serum 3.53 (2.07–4.85) 6.03 (3.93–9.73) 0.066
$Free T (pmol/L) FF 4.24 (1.55–7.87) 7.5 (5.28–11.74) 0.050*
$Bio-T (nmol/L) Serum 0.82 (0.48–1.14) 1.09 (0.8–1.9) 0.033*
$Bio-T (nmol/L) FF 0.99 (0.58–1.84) 1.75 (1.24–2.74) 0.050*
$FAI serum 3.03 (2.38–6.48) 6.03 (3.93–9.73) 0.035*
$FAI FF 3.7 (2.32–7.17) 6.93 (5.48–13.42) 0.050*
  1. Data are represented as median (inter-quartile range) for anthropometric and hormonal characteristics compared been controls and women with PCOS using Mann-Whitney U tests. Parameters marked with asterisk (#) denote those measured between days 3–5 of the menstrual cycle (early follicular phase) before initiating the controlled ovarian hyperstimulation (COH) procedure. Parameters marked by “$” were measured in sera and follicular fluids obtained on the day of ovum pick up (d-OPU). Menstrual characteristics were assessed using the chi-square analysis. P values < 0.05 are considered significant for all statistical tests. *P <  0.05, **P <  0.01, ***P </= 0.0001 have been indicated. BMI body-mass index, E2 estradiol, P4 progesterone, TT total testosteronem, SHBG sex hormone binding globulin, Bio-T bioavailable testosterone, Free-T free testosterone, FAI free androgen index.