Fig. 9

RFX1 recruits DNMT1, HDAC1, and SUV39H1 to the promoter region of TLR4 gene. a–d The enrichments of RFX1 (a), DNMT1 (b), HDAC1 (c), and SUV39H1 (d) in TLR4 promoter with RFX1 binding region were measured by ChIP-qPCR in CD14⁺ monocytes transfected with RFX1-shRNA or Cntl-shRNA. The fold changes were shown between RFX1-shRNA-1 group and Cntl-shRNA group. e–h The enrichments of RFX1 (e), DNMT1 (f), HDAC1 (g), and SUV39H1 (h) in TLR4 promoter with RFX1 binding region were measured by ChIP-qPCR in CD14⁺ monocytes transfected with RFX1-lentivirus or Cntl-lentivirus. The fold changes were shown between RFX1-lentivirus group with Cntl-lentivirus group. All values are the average of at least three biological replicates, and the data shown are the means ± SDs. *P < 0.05; **P < 0.01 relative to control