Fig. 7

RFX1 overexpression reversed the LDL induced epigenetic modification changes of the TLR4 promoter. a The percentage change of DNA methylation of four CG pairs (from − 1086 bp to − 421 bp upstream of the TLR4 TSS) was detected by bisulfite sequencing in CD14⁺ monocytes transfected with RFX1-lentivirus compared with Cntl-lentivirus (/RFX1-lentivirus). b The percentage change of mean DNA methylation of all the four CG pairs (from − 1086 bp to − 421 bp upstream of the TLR4 TSS) was detected by bisulfite sequencing in CD14⁺ monocytes transfected with RFX1-lentivirus compared with Cntl-lentivirus (/RFX1-lentivirus). c–e The fold changes of H3 (c), H4 (d) acetylation, and the H3K9 trimethylation (e) in TLR4 promoter with RFX1 binding region were detected by ChIP-qPCR in CD14⁺ monocytes transfected with RFX1-lentivirus compared with Cntl-lentivirus. All values are the average of at least three biological replicates, and the data shown are the means ± SDs. *P < 0.05; **P < 0.01 relative to control