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Fig. 6 | Clinical Epigenetics

Fig. 6

From: RFX1 downregulation contributes to TLR4 overexpression in CD14+ monocytes via epigenetic mechanisms in coronary artery disease

Fig. 6

Epigenetic modifications of the TLR4 promoter in CD14+ monocytes with RFX1 knockdown. a The percentage change of DNA methylation of four CG pairs (from − 1086 bp to − 421 bp upstream of the TLR4 TSS) was detected by bisulfite sequencing in CD14+ monocytes transfected with RFX1-shRNA-1 compared with Cntl-shRNA (/Cntl-shRNA). b The percentage change of mean DNA methylation of all the four CG pairs (from − 1086 bp to − 421 bp upstream of the TLR4 TSS) was detected by bisulfite sequencing in CD14+ monocytes transfected with RFX1-shRNA-1 compared with Cntl-shRNA (/Cntl-shRNA). c–e The fold changes of H3 (c), H4 (d) acetylation, and the H3K9 trimethylation in TLR4 promoter with RFX1 binding region were detected by ChIP-qPCR in CD14+ monocytes transfected with RFX1-shRNA-1 compared with Cntl-shRNA. All values are the average of at least three biological replicates, and the data shown are the means ± SDs. *P < 0.05; **P < 0.01 relative to control

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