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Table 3 PCR conditions for amplifying BSgDNA

From: Increased methylation upstream of the MEG3 promotor is observed in acute myeloid leukemia patients with better overall survival

Locus 1st PCR condition 2nd PCR condition COBRA
Polymerase Anealing temperature (°C) Cycles Polymerase Anealing temperature (°C) Cycles Restriction enzyme
IGF2-H19 DMR AmpliTaq 55 35 AmpliTaq 55 35 BstUI
DLK1-MEG3 IG-DMR (CG6) AmpliTaq 55 35 AmpliTaq 55 35 BstUI
MEG3 DMR (CG7) GoldTaq 57 40 GoldTaq 57 40 TaqI
MEG3 promotor (CG9) AmpliTaq 57 35 AmpliTaq 57 35 TaqI
MEG3 intragenic (CG8) AmpliTaq 55 35 AmpliTaq 55 35 BstUI
ZAC DMR GoldTaq 55 35 GoldTaq 55 38 BstUI
PEG1 DMR AmpliTaq 55 35 AmpliTaq 55 38 TaqI
PEG3 DMR AmpliTaq 55 35 AmpliTaq 55 35 BstUI