Table 3 PCR conditions for amplifying BSgDNA
Locus | 1st PCR condition | 2nd PCR condition | COBRA | ||||
|---|---|---|---|---|---|---|---|
Polymerase | Anealing temperature (°C) | Cycles | Polymerase | Anealing temperature (°C) | Cycles | Restriction enzyme | |
IGF2-H19 DMR | AmpliTaq | 55 | 35 | AmpliTaq | 55 | 35 | BstUI |
DLK1-MEG3 IG-DMR (CG6) | AmpliTaq | 55 | 35 | AmpliTaq | 55 | 35 | BstUI |
MEG3 DMR (CG7) | GoldTaq | 57 | 40 | GoldTaq | 57 | 40 | TaqI |
MEG3 promotor (CG9) | AmpliTaq | 57 | 35 | AmpliTaq | 57 | 35 | TaqI |
MEG3 intragenic (CG8) | AmpliTaq | 55 | 35 | AmpliTaq | 55 | 35 | BstUI |
ZAC DMR | GoldTaq | 55 | 35 | GoldTaq | 55 | 38 | BstUI |
PEG1 DMR | AmpliTaq | 55 | 35 | AmpliTaq | 55 | 38 | TaqI |
PEG3 DMR | AmpliTaq | 55 | 35 | AmpliTaq | 55 | 35 | BstUI |