Fig. 4

Differential histone methylation in Msh2^−/− compared to Msh2^+/+ mice. a Comparison of the histone modification state distributions for differentially modified genes (10,767 genes). b Transition matrices for the five most common histone modification states. The numbers of genes contributing to the individual transitions are given. The selected state transitions refer to 68% of the differentially modified genes. Notably, each gene below the diagonal becomes epigenetically activated, while genes above the diagonal become epigenetically repressed. c ChIP-qPCR for genes of the Set1^−/− transition gene sets validating their H3K36me3 recruitment in Msh2^−/− mice. All PCR results were normalized to input controls and to an unmodified gene region (mean ± SD, n = 4). d Boxplot of the average expression of Set1^−/− genes. Set1^−/− genes show an intermediate expression between stable [100] and stable [101] modified genes. No expression differences are detected for all sets between Msh2^+/+ and Msh2^−/− mice