Fig. 2

Identification and validation of gene-associated histone modification peaks. a Barplots of the total number of quality peaks and the fraction of these peaks associated with genes (colored fraction) for each modification and Msh2 genotype (K4: H3K4me3, K27: H3K27me3, K36: H3K36me3). b–d Upper row: screenshots of the H3K4me3, H3K27me3, and H3K36me3 signals from the ChIP-seq analysis displayed in the UCSC Genome Browser (https://genome.ucsc.edu/) at selected genes (position indicated by red arrows) for Msh2^+/+ mice. The peak regions are depicted as black bars below the signals. Lower row: ChIP-qPCR for selected genes performed on Msh2^+/+ replicates. All PCR results were normalized to input controls and to an unmodified gene region (mean ± SD, n = 4)