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Table 2 Primer sequences and optimal MS-HRM conditions of OPCML, HOXA9, and HOXD9

From: Serum cell-free DNA methylation of OPCML and HOXD9 as a biomarker that may aid in differential diagnosis between cholangiocarcinoma and other biliary diseases

Gene

Primer

Primer sequence 5′- > 3′

UCSC genome browser (December, 2013)

Product (bp)

MgCl2 (mM)

Ta (°C)

OPCML

F

CGATCGGGTTGTAGAGGA

chr11: 132943630–132943731

101

2.5

63

R

CGCATCTAAAACCCCAAAAC

HOXA9

F

AATGCGATTTGGTTGTTTTTTT

chr7: 27165810–27165963

153

2.5

63

R

CCCCATACACACACTTCTTAAAC

HOXD9

F

GATCGAGGGTTGTAAGAAGAAG

chr2: 176122435–176122541

106

2.5

65

R

CCCGACCTAACCTAACCC

  1. Ta annealing temperature