Fig. 2From: Targeted design and identification of AC1NOD4Q to block activity of HOTAIR by abrogating the scaffold interaction with EZH23D HOTAIR modeling prediction and identification of AC1NOD4Q as a potent compound interfering the HOTAIR/EZH2 interaction. a 3D RNA modeling of 212–300 nt in the 5′ functional domain of HOTAIR that binds to EZH2 was conducted by the MC-Fold and MC-Sym programs. b Screening process to identify lead compounds that specifically abrogate scaffold interactions between the PRC2 complex and HOTAIR. c Chemical structure of ADQ. d ADQ treatment had the strongest effect on NLK luciferase activation in LN229, U87, and U87 EGFRvIII glioma cells and MDA-MB-231 breast cancer cells. e NLK mRNA levels were measured in various cell lines via qRT-PCR after treatment with ADQ. f HOTAIR expression was detected in DMSO- and ADQ-treated cellsBack to article page