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Fig. 2 | Clinical Epigenetics

Fig. 2

From: Tracking of epigenetic changes during hematopoietic differentiation of induced pluripotent stem cells

Fig. 2

Comparison of DNA methylation profiles of iPSC-derived HPCs versus primary CD34+ cells. a Scatter plot of CpG sites that are either higher methylated (2692 CpG sites, red) or less methylated in iHPCs (2307 CpG sites, blue; delta mean β value > 0.5 or < − 0.5) compared to primary CD34+ cells. CpG sites associated with promoter regions are highlighted in bold (659 CpG sites and 587 CpG sites, respectively). b Gene ontology analysis of genes with differentially methylated CpG sites in the promoter region. Enrichment of specific categories was calculated by the one-sided Fisher’s exact P value. c DNA methylation patterns within the genes CD45 and CD34. Mean DNAm levels are depicted for iPSCs (GSE37066), iHPC d20, and primary CD34+ cells. TSS1500: 1500 bp upstream of transcription start site; UTR: untranslated region. For all analyses, iHPCs were compared to primary CD34+ cells isolated from human cord blood (GSE40799)

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