Table 3 Selected regions, reference, location, PCR amplification primers, PCR template size, pyrosequencing primers, and number of CpG sites analyzed
Gene symbol Ensembl accession number | Selected regions Start-End | PCR Primer sequences 5′-3′ (bp) | PCR product size (bp) | Pyrosequencing primer sequences 5′- 3′ (bp) | CpG sites included |
|---|---|---|---|---|---|
Leptin (LEP) ENSG00000174697 | Chr7: 128,241,050 Chr7: 128,241,412 | F: ATTTTTGGGAGGTATTTAAGGG R: ACATCCCTCCTAACTCAATTT | 362 | Pyro1: GTTATTTTGAGGGG Pyro2: TTATAAGAGGGG | #17 |
Adiponectin (ADIPOQ) ENSG00000181092 | Reg1: Chr3: 186783987 Chr3: 186,784,378 | F: GGAGGGTTTTAGGTTTTATTTG R: ACCACCTCAACTACACCTTAAA | 391 | Pyro1: TGGGGAAGGGTTGGAGGTA Pyro2: GTATTGTTGGGGG | #21 |
Reg2: Chr3: 186801089 Chr3: 186,801,356 | F: GTTTTTTTGGAGAGGAGAGAAAG R: CCRCAAAAAAAACAACTCTC | 267 | Pyro1: TTTAGGGAGAAAAAGAAGA Pyro2: GTAGTATTAAGAAGGAG | #12 | |
Leptin receptor (LEPR) ENSG00000116678 | Chr1: 65,420,597 Chr1: 65,420,959 | F: TTTGGTTTGGGTAGGTTGT R: AAAAAAACCAAAACTCCCC | 288 | Pyro1: TTTGGTTTGGGTAGGTTGT Pyro2: GTTAAAGGTATAT | #13 |
Adiponectin receptor 1 (ADIPOR1) ENSG00000159346 | Chr1: 202,957,739 Chr1: 202,958,035 | F: TGGTAATTTAATGYGGTTGTT R: CCTAACCTCCAAACATCCA | 299 | Pyro1: TGGTAATTTAATGYGGTTGTT Pyro2: GTAGTATTTATAGGATTGT | #21 |
Adiponectin receptor 2 (ADIPOR2) ENSG00000006831 | Chr12: 1,691,140 Chr12: 1,691,555 | F: TAGYGGTGGTTTTTAAGAAGT R: AACTAAAACAACTACACCCRAA | 379 | Pyro1: TAGYGGTGGTTTTTAAGAAGT Pyro2: AGGTGAGAGTTGAGGGG | #16 |