Fig. 2From: Whole-genome methylation profiling of the retinal pigment epithelium of individuals with age-related macular degeneration reveals differential methylation of the SKI, GTF2H4, and TNXB genesBisulfite pyrosequencing of candidate gene SKI (cg18934822) validates direction of methylation change identified in 450k array. a–c Univariate analysis of SKI (cg18934822) in Illumina 450k BeadChip array. a Methylation β values for AMD (n = 25) compared to normal (n = 19) donor RPE cells after normalization, in addition to analysis of sex-stratified results for cg18934822 (SKI). Significantly reduced methylation levels are observed in AMD (p < 0.0001), as well as in both AMD male (n = 15) (b) and AMD female (n = 10) (c) compared to normal male (n = 12) (p = 0.0063) and normal female (n = 7) (p = 0.0031) human RPE donor cells respectively. d–f Bisulfite pyrosequencing of candidate gene SKI (cg18934822) in combined technical and independent sample replications. d Reduced methylation of cg18934822 (SKI) is identified in AMD (n = 30) compared to normal donor RPE cells (n = 25) (p = 0.0309). e Reduced methylation of cg18934822 (SKI) is identified in AMD AMD male (n = 20) compared to normal male (n = 18) (p = 0.1223) and f AMD female (n = 10) compared to normal female donor samples (n = 7) (p = 0.1002). (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001). All statistical analysis was performed using the Mann-Whitney U testBack to article page