Fig. 3
From: Elevated H3K27me3 levels sensitize osteosarcoma to cisplatin
Increasing H3K27me3 levels sensitizes OS to cisplatin in vitro. a The viability of 143B and HOS cells without treatment and after treatment with EPZ (10 μM) or GSK-J4 (15 μM) for 72 h was analyzed by CCK-8 assay. b The percentage of apoptotic cells among 143B and HOS cells without treatment and after treatment with EPZ (10 μM) or GSK-J4 (15 μM) for 48 h was analyzed by flow cytometry. c Immunofluorescence of H3K27me3 (green) and γH2AX (red) in 143B cells subjected to the indicated treatments for 24 h. d H3K27me3 and γH2AX expression in OS cells subjected to the indicated treatments was analyzed by western blot analysis. * P < 0.05, **P < 0.01. Abbreviation: IOD, integrated optical density