Fig. 5From: TRIM4 is associated with neural tube defects based on genome-wide DNA methylation analysisPyrosequencing to identify methylated CpG sites in TRIM4. DNA methylation levels at each CpG site (POS) by percentage were analyzed by pyrosequencing in NTDs (black bar) and controls (gray bar) in exon 1 (a, b), promoter region (c), and both regions (d). Data are represented as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. controlsBack to article page