Fig. 4

Deregulated pathways and candidate target genes of miR-199a-3p. a Transcriptomic analysis of WM-266-4 GFP cells transfected with miR-199a-3p identified differentially expressed genes with a FC > 1.5. Heatmaps represented all deregulated genes for the two conditions (0.25 and 0.5 nM of miR-199a-3p). P value cutoff is FDR corrected. b Venn-diagram of all downregulated genes with a FC > 1.5 for miR-199a-3p-transfected cells (812 genes), crossed with its predicted targets (common to five different softwares). The table summarises the 13 retrieved genes. Genes in bold were further analysed. c CD2AP, FOS, ITGB8, NEDD4, SERPINE2 and VAMP3 expression in WM-266-4 cells transfected with miR-199a-3p or treated with 5azadC. The mean fold inhibition was calculated as the ratio of RQ in treated cells/RQ in the control for each gene. SEM are shown for triplicates. *P value < 0.05, **P value < 0.01. NS not significant