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Table 6 Summary of the immunoreactivity stability of histone acetylation, histone methylation, and DNA cytosine modifications in brain samples*

From: DNA methylation and histone post-translational modification stability in post-mortem brain tissue

Epigenetic modification IHC IHC—immunoreactivity IHC—proportion IHC—counts Western blot
Human—Cx Pig—Cx Mouse—DG Pig—Cx Mouse—DG Neurons Glia Pig—Cx
5mC 120 72 72 72 72 72 72 n/a
5hmC 120 72 72 72 72 n/a
5fC 96 72 72 72 72 n/a
5caC 96 72 72 72 72 n/a
H3K4me3 72 72 72 72 72 72
H3K9me2/K9me3 nd** 72 72 72 72 nd** nd** No signal**
H3K27me3 72 72 72 72 72 72 72 72
H3K36me3 72 72 72 72 72 48
H3K9ac 72 48 48 24 24 48 72 Wrong mw
H3K14ac 53 72 72 72 72 72 48 Low signal
H3K27ac 53 24 24 24 24 72 72 Wrong mw
H4K5ac 72 72 48 24 < 24 24 48 < 24
H4K12ac 120 24 24 72 72 72 72 24
H4K16ac nd 24 24 24 < 24 72 48 No signal
Total H3 nd 24 72 48 48 72 h
Total H4 nd 72 72 72 48 72 h
H3panAc 72 nd nd nd nd nd nd 24 h
  1. IHC immunohistochemistry, Cx neocortex, DG dentate gyrus of hippocampus, nd not done, n/a not applicable; not counted; mw molecular weight *Numeric values represent hours post-mortem to formalin fixation (or freezing for Western blots) at which there are no differences compared to samples that were fixed or frozen immediately after death
  2. **H3K9me2,K9me3 antibody was discontinued; H3K9me2 replacement antibody could not be optimized