Table 2 Summary of dot blot evaluation of antibody specificity
From: DNA methylation and histone post-translational modification stability in post-mortem brain tissue
Peptide | Antibody | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Total H3 | Total H4 | H3K27ac | H3K14ac | H4K5ac | H4K12ac | H3panAc | H3K4me3 (Abcam) | H3K4me3 (Active Motif) | H3K27me2 | H3K27me3 | H3K36me3 | |
Histone mix | Strong | None | ||||||||||
Unmodified H3 | Strong | None | None | None | None | [Weak] | None | |||||
Unmodified H4 | None | [None] | None | None | ||||||||
H3K4me | [Weak] | [Weak] | ||||||||||
H3K4me2 | [Weak] | [Strong] | [Weak] | |||||||||
H3K4me3 | Strong | Strong | [Weak] | |||||||||
H3K9me | None | |||||||||||
H3K9me2 | None | [Weak] | ||||||||||
H3K9me3 | [Weak] | [Weak] | None | |||||||||
H3K27me | None | None | ||||||||||
H3K27me2 | [Weak] | None | ||||||||||
H3K27me3 | None | [Weak] | [Strong] | Strong | Strong | [Strong] | ||||||
H3K36me | None | |||||||||||
H3K36me2 | [Weak] | [Strong] | ||||||||||
H3K36me3 | [Weak] | None | Strong | |||||||||
H3K14ac | None | Strong | None | None | Strong | |||||||
H3K27ac | None | None | Strong | None | None | None | Weak | |||||
H4K5ac | None | None | Strong | [Weak] | None | |||||||
H4K12ac | None | None | None | None | Strong | None | ||||||
Summary | Specific | Specific* | Specific | Specific | Specific | Fairly specific | Specific | Fairly specific | Not very specific | Fairly specific | Specific | Fairly specific |