Fig. 4
From: DNA methylation and histone post-translational modification stability in post-mortem brain tissue
Immunohistochemical detection of 5 methylcytosine (5mC) in mouse and neonatal pig neocortex. Labeling is strongest in the large round nuclei of neurons in mouse brain; note that immature neurons in newborn mouse are usually negative. In pig brain, smaller glial nuclei are also labeled. Elongated nuclei of endothelial cells are generally negative. There is no difference between 0 (left) and 72 (right) hours post-mortem in any of the animal groups. Images taken at × 400 magnification. DAB detection of antibody (brown) and hematoxylin counterstain (blue)