Fig. 2

The combination of MPT0G211 and doxorubicin had a synergistic effect on HL-60 cell death. a The viability of cells treated with MPT0G211 and different doses of doxorubicin (DOXO), vincristine (VCR), and cyclophosphamide (CTX) for 48 h was tested. The synergistic effects of these drugs were evaluated using the combination index (CI), with values of > 1.0, 1.0, and < 1.0 indicating an antagonistic, additive, or synergistic interaction, respectively. b Cell cycle distribution was determined in cells treated with various concentrations of MPT0G211 and DOXO for 48 h. c The combined effect of MPT0G211 and DOXO on the expression of caspases 3, 8, and 9 and poly-ADP ribose polymerase (PARP). d Flow cytometry was used to evaluate the proportion of cells in the sub-G1 phase of cell cycle following incubation with 3 μM MPT0G211 or tubastatin A (TBA) combined with 0.1 μM DOXO. Data are shown as means ± standard errors of the means. *p < 0.05, **p < 0.01, and ***p < 0.001 versus the control group