Significantly altered peripheral blood cell DNA methylation profile as a result of immediate effect of metformin use in healthy individuals

Table 2 Characterization of the top 11 most significant CpG sites

Filter	CpG site	Chr	logFC M10h vs M0	logFC M7d vs M0	FDR M10h vs M0	FDR M7d vs M0	Gene	Distance to the closest TSS	Gene context^a	Spearman’s correlation between methylation and transcription
Significant in both of the following contrasts: M10h vs M0 and M7d vs M0	cg03515060	21	0.538	0.479	0.003	0.018	POFUT2	1984	Body	− 0.184
	cg18394557	17	0.406	0.286	0.003	0.047	CAMKK1	− 8799	Body	− 0.042
	cg16013966	11	0.428	0.395	0.034	0.037	EML3	− 308	1stExon;5’UTR;TSS1500	− 0.23
	cg05638165	1	0.358	0.351	0.035	0.034	KIAA1614	14,198	Body	− 0.337
	cg18824330	19	0.363	0.419	0.043	0.022	UPF1	− 9944	Body	− 0.382
Significant in one of the contrasts and medians for time points M10h or M7d in IQR	cg12740863	3	− 0.359	− 0.260	0.034	0.127	MUC4	− 26,158		0.37
	cg16843994	2	0.347	0.404	0.091	0.038	LOC727982	− 706		NA
	cg12162450	2	− 0.386	− 0.349	0.040	0.059	SIX3	7515		NA
	cg19176072	10	0.462	0.472	0.054	0.040	ADAM8	5756	Body	− 0.312
	cg01644741	20	− 0.287	− 0.366	0.137	0.043	SNORD12B	39	Body,TSS1500	0.036
	cg02622542	3	0.269	0.348	0.151	0.047	VPS8	− 2419		0.166

ISSN: 1868-7083