Fig. 2

Snail2 induces migration and metastasis in CSC cells, but does not promote cell proliferation. a The migration of SW480 cells (overexpressing Snail2 or control) was analyzed by wound healing assays. The statistical analysis is shown in the bar graph (mean ± SD from three independent experiments), and a representative experiment is shown in the right panel. Phase contrast images were taken at × 4 magnification. b The invasiveness of SW480 cells (overexpressing Snail2 or control) was analyzed in invasion assays. The fold change in invasion is shown in the bar graph (mean ± SD from three independent experiments), and a representative experiment is shown in the left panel. Phase contrast images were taken at × 10 magnification. c Proliferation of SW480-Snail2 and control cells was examined using the CCK-8 assay Kit. d Apoptosis analysis, caspase3, Bcl2, PARP, and PARP cleavage was examined in SW480-N and SW480-Snail2 by western blotting. e SW480 cells (overexpressing Snail2 or control) were subcutaneously injected into nude mice. Each group had six mice. After 30 days, the mice were sacrificed and the liver was dissected. Liver metastatic nodules were examined macroscopically or paraffin-embedded, cut into sections, and subjected to H&E and immunohistochemical staining. Immunohistochemical staining of Ki-67 expressed in liver tissues. (Arrowheads indicate liver metastases). Phase contrast images were taken at × 40 magnification. f The total number of mice with distant metastasis at 30 days after injection of Snail2-overexpressing or control SW480 cells. g Weight of tumors following subcutaneous injection of Snail2-overexpressing or control SW480 cells