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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Differential open chromatin profile and transcriptomic signature define depot-specific human subcutaneous preadipocytes: primary outcomes

Fig. 3

Promoter accessibility of depot-specific genes. a Schematic representation of the investigation of promoter accessibility using ATAC-seq. ATAC-seq signals were calculated around the transcription start sites (TSSs) of the depot-specific genes in a 500-bp-wide window (250 bp up- (5′) and downstream (3′) relative to the TSSs). b Histogram-based depiction of the average tag density at the promoter regions of abdominal- (n = 126) and GF-specific (n = 90) genes in a 1-kb window centered on the TSSs. ATAC-seq density around the TSS of abdominal and GF depot-specific genes in each depot and for each subject (DiffBind). Sub1 = Subject 1, Sub2 = Subject 2 paired t test; **p < 0.01, ***p < 0.005. c The number of genes with open promoter in abdominal (red) or GF (green) depot, according to their expression specificity (on the left side are the genes upregulated in abdominal preadipocytes, on the right side are the genes upregulated in GF preadipocytes). The gray bars represent the genes with no differential opened chromatin between A and GF depot

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