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Table 3 Pyrosequencing for the relative quantitation of CpG methylation in CD14 and HLA-DOA genes

From: Two histologically colorectal carcinomas subsets from the serrated pathway show different methylome signatures and diagnostic biomarkers

Gene   CD14 HLA-DOA
CpG site   CpG1 CpG2 CpG1 CpG2 CpG3
SAC-T (n = 16) Mean 6.89 13.56 33.83 34.95 89.78
  SD 5.53 8.06 15.47 14.91 8.04
hMSI-H-T (n = 9) Mean 13.64 22.38 50.94 48.12 94.61
  SD 6.15 8.9 14.81 13.62 2.36
SAC-T vs. hmMSI-H p value 0.010 0.020 0.013 0.039 0.14
SAC-N (n = 8) Mean 7.97 13.86 60.02 58.27 100
  SD 3.19 2.19 2.01 2.94 0
MSI-N (n = 6) Mean 8.25 13.72 53.13 53.26 98.69
  SD 2.36 2.37 6.73 8.92 2.75
SAC-T vs. SAC-N p value 0.616 0.911 0.0001 0.0003 0.006
hmMSI-H-T vs. hmMSI-H-N p value 0.064 0.038 0.742 0.432 0.002
SAC-N vs hmMSI-N p value 0.860 0.911 0.017 0.159 0.271
  1. SAC-T tumoural tissue from serrated adenocarcinoma, hmMSI-H-T tumoural tissue from histological and molecular high-level microsatellite unstable colorectal carcinoma, SAC-N normal tissue adjacent to SAC, hmMSI-H-N normal tissue adjacent to hmMSI-H