Table 1 Clinico-pathological features of the study cases
Training set | DNA validation set (pyrosequencing) | RNA validation set (qPCR) | Protein validation set (IHC) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
SAC | hmMSI-H | SAC | hmMSI-H | SAC | hmMSI-H | SAC | hmMSI-H | |||||
n = 39 (%) | n = 9 (%) | p value | n = 16 (%) | n = 9 (%) | p value | n = 19 (%) | n = 22 (%) | p value | n = 26 (%) | n = 21 (%) | p value | |
Gender | ||||||||||||
Female | 20 (51.3) | 7 (77.8) | 8 (50) | 7 (77.8) | 15 (78.9) | 14 (63.6) | 19 (73.1) | 12 (57.1) | ||||
Male | 19 (48.7) | 2 (22.2) | 0.284 | 8 (50) | 2 (22.2) | 0.174 | 4 (21.1) | 8 (36.4) | 0.234 | 7 (26.9) | 9 (42.9) | 0.201 |
Age [SD] | 71.6 [10.0] | 66.4 [15.22] | 0.094 | 72.0 [9.5] | 70.3 [8.4] | 0.267 | 69.0 [8.43] | 69.75 [13.9] | 0.839 | 69.9 [6.8] | 70 [10.7] | 0.969 |
Location | ||||||||||||
Proximal | 26 (66.7) | 8 (100) | 9 (56.3) | 8 (88.9) | 10 (52.6) | 15 (68.2) | 14 (53.8) | 13 (61.9) | ||||
Distal/rectum | 13 (33.3) | 1 (0) | 0.182 | 7 (43.8) | 1 (11.1) | 0.107 | 9 (47.4) | 7 (31.8) | 0.309 | 12 (46.2) | 8 (38.1) | 0.399 |
Dukes’ stage | ||||||||||||
A | 4 (10.3) | 1 (11.1) | 2 (12.5) | 1 (11.1) | 3 (15.8) | 6 (27.3) | 4 (15.4) | 4 (19.0) | ||||
B | 13 (33.3) | 5 (55.6) | 7 (43.8) | 5 (55.6) | 7 (36.8) | 9 (40.9) | 9 (34.6) | 10 (47.6) | ||||
C | 17 (43.6) | 3 (33.3) | 0.846 | 7 (43.8) | 3 (33.3) | 0.846 | 9 (47.4) | 7 (31.8) | 0.522 | 13 (50.0) | 7 (33.3) | 0.523 |
WHO grade | ||||||||||||
High | 5 (12.8) | 0 (0) | 1 (6.3) | 0 (0) | 1 (5.3) | 0 (0) | 0 (0) | 0 (0) | ||||
Low | 34 (87.2) | 9 (100) | 0.596 | 15 (93.8) | 9 (100) | 0.64 | 18 (94.7) | 22 (100) | 0.463 | 26 (100) | 21 (100) | NA |
Type | ||||||||||||
Non-mucinous | 34 (87.1) | 6 (66.7) | 14 (87.5) | 6 (66.7) | 17 (89.5) | 19 (86.4) | 22 (84.6) | 18 (85.7) | ||||
Mucinous | 5 (12.8) | 3 (33.3) | 0.321 | 2 (12.5) | 3 (33.3) | 0.23 | 2 (10.5) | 3 (13.6) | 0.572 | 4 (15.4) | 3 (14.3) | 0.623 |