Prediction of smoking by multiplex bisulfite PCR with long amplicons considering allele-specific effects on DNA methylation

Table 1 Amplicons utilised in the study

Reference (index) CpG, Illumina ID	The closest gene to the reference CpG	Genome coordinate (hg19) of the amplicon	DNA strand of the amplicon	Length of the amplicon, bp	Amount of CpGs in the amplicon	Reference
cg05575921	AHRR	chr5:372478-373819	+	1342	44	[28, 29, 63,64,65,66,67,68,69,70]
cg21566642	ALPPL2	chr2:233283630-233284930	−	1301	114	[28, 29, 63,64,65,66, 68, 70]
cg06126421	IER3	chr6:30719327-30720645	+	1319	19	[28, 29, 65, 66, 68, 70]
cg25189904	GNG12	chr1:68298855-68300158	−	1304	85	[28, 29, 64, 68, 69]
cg09935388	GFI1	chr1:92947265-92948622	+	1358	73	[28, 29, 67, 68, 70]
cg15417641	CACNA1D	chr3:53699512-53700811	−	1300	17	[28, 29, 68]

ISSN: 1868-7083