Fig. 1

Methylation analysis. a Methylation screening of HL60 cell line. b DAPK1 methylation-specific polymerase chain reaction analysis. HL60 cells treated with 50 and 75 μmol/L Qu for 48 and 72 h and 1 and 2 μmol/L 2-deoxy-5-aza cytidine for 72 h. Lane L: ladder; lane M: amplified product with primers for methylated sequences (106 bp); lane U: amplified product with primers for unmethylated sequences (98 bp). c BCL2L11 methylation-specific polymerase chain reaction analysis. HL60 and U937 cells treated with 50 and 75 μmol/L Qu for 48 and 72 h and 2 μmol/L 2-deoxy-5-aza cytidine for 72 h. L: ladder; lane M: amplified product with primers for methylated sequences (139 bp); lane U: amplified product with primers for unmethylated sequences (139 bp). d Bisulfite sequencing of DAPK1 promoter: original DNA sequence, bisulfite-modified DNA sequence (methylated), and bisulfite-modified DNA sequence (unmethylated). e Electropherogram for HL60 cell line. f Electropherogram for HL60 treated with 75 μmol/L Qu for 72 h. Y represents heterozygote C/T double peaks, indicating partial methylation