Fig. 4

Potential function of SUV39H2 in lung adenocarcinoma cell lines. a, b A549 cells were transfected with siControl or siRNA targeting SUV39H2, before being seeded into the transwell chambers. The invaded cells were stained and counted, and the images represent one field under the microscope (× 10 magnification). The error bars represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01 (two-tailed unpaired t test). For each complete experiment, three independent samples were subjected to analysis in the indicated group, and every experiment was conducted in triplicate. c A549 cells infected with the indicated lentiviruses were maintained in culture media for 5 days prior to being stained with crystal violet. Representative photos are shown on the left; they were statistically analyzed as shown on the right. For each complete experiment, three independent samples were analyzed in the indicated group, and every experiment was conducted in triplicate. d A549 cells infected with lentiviruses expressing shSCR or shSUV39H2 were inoculated subcutaneously in 6-week-old female nude mice (n = 6). Six tumors were quantified using bioluminescence imaging 1 week after the initial implantation. The error bars indicate the mean ± SD. *p < 0.05, **p < 0.01 (two-tailed t test). e A549 cells infected with lentiviruses expressing shSCR of shSUV39H2 were injected intravenously in 6-week-old female SCID mice (n = 3). The tumors were detected via bioluminescence imaging. f The knockdown efficiency was confirmed by RT-qPCR and Western blotting