Fig. 2

SUV39H2 overexpression in lung cancer tissues and lung cancer cell lines potentially influenced the expression of a variety of genes. a The globular proteins of eight surgically removed tumor tissues and the adjacent normal tissues were extracted, and the expression of SUV39H2 was assessed via Western blotting, with GAPDH serving as the internal control. b The whole-cell lysates of the indicated cell lines were used in the Western blot analysis to examine the expression of SUV39H2; GAPDH served as the control. c The representative image of SUV39H2 stain in indicated tissue sample of TMA and the percentage of SUV39H2-positive cells in each group are presented. d, e qRT-PCR and Western blotting were utilized to verify the knockdown of SUV39H2 at both the mRNA and protein levels. Each sample with the indicated primers was used for the PCR analyses in three replicates, and the experiment was performed in triplicate. f Heatmap of the representative genes identified in the RNA-seq. The samples were from three independent transfections with three different siRNA sequences