Fig. 3

DNA methylation age acceleration variation across preprocessing methods. a Scatter plot of EPIC DNA methylation (DNAm) age calculated from raw and data from three different preprocessing methods: quantile, GenomeStudio (GS), and normal exponential out-of-band (noob) normalization. Colored regression lines and surrounding shaded gray areas represent 95% confidence interval for each group. b Boxplot of estimated DNA methylation (DNAm) age—chronological age (acceleration difference) for each preprocessing method. c Boxplot of residuals from a linear regression (DNAm age~chronological age) across methods. The median is illustrated by horizontal line with upper and lower hinges representing the 25th and 75th percentiles, upper and lower whiskers extend no further than the inter-quartile range multiplied by 1.5. Colored data points represent individual samples for each group