Fig. 2

EZH2 inhibition differentially affects MMCs survival. a EPZ-6438 treatment leads to MM cell growth inhibition and apoptosis induction. HMCLs were exposed to different doses of EPZ-6438 and cell viability was analyzed after 4, 8, 11, and 15 days. Cells were split, replated, and treated at each time points. Results are the mean absolute cumulated counts ± SD of viable myeloma cells of five independent experiments. Cell cycle of EPZ-6438-treated MM cell lines was analyzed by flow cytometry. Results are representative of four independent experiments. Apoptosis induction was analyzed with Annexin V PE staining by flow cytometry. The shown data are the mean values ± SD of four separate experiments. * indicates a significant difference compared to control cells using a Wilcoxon test for pairs (P ≤ 0.05). b EZH2 inhibition induces mortality of primary MMCs from patients. At day 8 of culture, the viability and total cell counts were assessed, and the percentage of CD138 viable PC was determined by flow cytometry. Results are median values of the numbers of myeloma cells in the culture wells. c Global H3K27me3 status was also assessed by flow cytometry. Results are median values of the H3K27me3 staining index in CD138 viable PC. * indicates a significant difference compared to control cells using a Wilcoxon test for pairs (P ≤ 0.05)