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Fig. 6 | Clinical Epigenetics

Fig. 6

From: CDX2 in colorectal cancer is an independent prognostic factor and regulated by promoter methylation and histone deacetylation in tumors of the serrated pathway

Fig. 6

HDAC5 regulates CDX2 expression by binding to the promoter region of the CDX2 gene. a Schematic representation of the genomic region upstream of the transcriptional start site (TSS) of the CDX2 gene. ChIP primer locations are indicated by arrows. b In vivo binding of HDAC5 to the indicated genomic regions upstream of the CDX2 TSS was shown by ChIP in 293T cells transfected with FLAG-tagged HDAC5 using antibodies against FLAG. Antibodies against acetyl-histone H3 and mouse IgG were used as positive and negative controls, respectively. Amplification of a genomic region in the FGF2 gene was shown as a positive control for a genomic region bound by HDAC5. *unspecific PCR band

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