Fig. 6From: CDX2 in colorectal cancer is an independent prognostic factor and regulated by promoter methylation and histone deacetylation in tumors of the serrated pathwayHDAC5 regulates CDX2 expression by binding to the promoter region of the CDX2 gene. a Schematic representation of the genomic region upstream of the transcriptional start site (TSS) of the CDX2 gene. ChIP primer locations are indicated by arrows. b In vivo binding of HDAC5 to the indicated genomic regions upstream of the CDX2 TSS was shown by ChIP in 293T cells transfected with FLAG-tagged HDAC5 using antibodies against FLAG. Antibodies against acetyl-histone H3 and mouse IgG were used as positive and negative controls, respectively. Amplification of a genomic region in the FGF2 gene was shown as a positive control for a genomic region bound by HDAC5. *unspecific PCR bandBack to article page