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Table 4 Mediation effects of DNA methylation levels on the association between rs174570 and gene expression

From: FADS1-FADS2 genetic polymorphisms are associated with fatty acid metabolism through changes in DNA methylation and gene expression

Outcome Total effect Model Direct effect Bootstrap analysis
β ± SE P 1   β ± SE P 2 Boot LLCI Boot ULCI
FADS1 mRNA level − 0.142 ± 0.040 0.001 Adjusted for CpG_9 − 0.019 ± 0.068 0.782 − 0.264 − 0.023
   Adjusted for CpG_10 − 0.066 ± 0.055 0.235 − 0.173 − 0.014
   Adjusted for CpG_11 − 0.115 ± 0.054 0.042 − 0.090 0.068
   Adjusted for CpG_2 0.002 ± 0.080 0.976 − 0.284 − 0.005
   Adjusted for CpG_3 0.043 ± 0.075 0.572 − 0.295 − 0.047
FADS2 mRNA level − 0.094 ± 0.037 0.016 Adjusted for CpG_9 0.002 ± 0.063 0.977 − 0.217 0.004
   Adjusted for CpG_10 − 0.038 ± 0.519 0.472 − 0.162 0.004
   Adjusted for CpG_11 − 0.055 ± 0.050 0.275 − 0.103 0.032
   Adjusted for CpG_2 0.042 ± 0.074 0.571 − 0.265 − 0.008
   Adjusted for CpG_3 0.066 ± 0.070 0.350 − 0.243 0.005
  1. P1 represents the total effect of rs174570 on FADS1 or FADS2 gene expression without adjustment for DNA methylation; P2 represents the effect of rs174570 on FADS1 or FADS2 gene expression controlling for DNA methylation. A mediation effect was considered significant when 0 was not included in the bootstrap confidence interval. The characters shown in italics refer to a significant mediation effect. Boot LLCI, Boot low limit confidence interval; Boot ULCI, Boot upper limit confidence interval; FADS, fatty acid desaturase