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Table 4 Mediation effects of DNA methylation levels on the association between rs174570 and gene expression

From: FADS1-FADS2 genetic polymorphisms are associated with fatty acid metabolism through changes in DNA methylation and gene expression

Outcome

Total effect

Model

Direct effect

Bootstrap analysis

β ± SE

P 1

 

β ± SE

P 2

Boot LLCI

Boot ULCI

FADS1 mRNA level

− 0.142 ± 0.040

0.001

Adjusted for CpG_9

− 0.019 ± 0.068

0.782

− 0.264

− 0.023

  

Adjusted for CpG_10

− 0.066 ± 0.055

0.235

− 0.173

− 0.014

  

Adjusted for CpG_11

− 0.115 ± 0.054

0.042

− 0.090

0.068

  

Adjusted for CpG_2

0.002 ± 0.080

0.976

− 0.284

− 0.005

  

Adjusted for CpG_3

0.043 ± 0.075

0.572

− 0.295

− 0.047

FADS2 mRNA level

− 0.094 ± 0.037

0.016

Adjusted for CpG_9

0.002 ± 0.063

0.977

− 0.217

0.004

  

Adjusted for CpG_10

− 0.038 ± 0.519

0.472

− 0.162

0.004

  

Adjusted for CpG_11

− 0.055 ± 0.050

0.275

− 0.103

0.032

  

Adjusted for CpG_2

0.042 ± 0.074

0.571

− 0.265

− 0.008

  

Adjusted for CpG_3

0.066 ± 0.070

0.350

− 0.243

0.005

  1. P1 represents the total effect of rs174570 on FADS1 or FADS2 gene expression without adjustment for DNA methylation; P2 represents the effect of rs174570 on FADS1 or FADS2 gene expression controlling for DNA methylation. A mediation effect was considered significant when 0 was not included in the bootstrap confidence interval. The characters shown in italics refer to a significant mediation effect. Boot LLCI, Boot low limit confidence interval; Boot ULCI, Boot upper limit confidence interval; FADS, fatty acid desaturase