Fig. 7

ID4 re-expression reduces ERα levels in MCF-7 cell lines. The figure represents the absolute quantification of ER expression (top left) and normalized respect to β-actin (right) by ddPCR. The fluorescence amplitude (y-axis) represents the intensity of amplification in each droplet, and each blue dot is a droplet in which the target has been amplified. To calculate the copies/droplet, a Poisson correction is performed which requires full (dost above the pink line) and empty droplets (dots below the horizontal pink line). For this, a minimum and equal amount of template cDNA (10 ng) is used for each condition. a With the ddPCR assay for ER expression, a variation was observed in the number of droplets with signal of ERα detection. β-actin was used as a control. Results are presented as copies per microliter in the amplification reaction. b Bar graph represents the mean of three technical experiments measuring the expression of ER in ID4-transformed MCF7 cells, by ddPCR. **p < 0.05 in comparison with the control per Student’s t test