Fig. 4

Analyses of distribution of H3K9me3 and H3K27me3 in the promoters of immune checkpoints/ligands in colorectal tumor and normal colon tissues. Cells from five individual NT and TT samples were isolated by enzyme disaggregation. Chromatin was precipitated using anti-H3 as control, anti-H3K9me3, anti-H3K27me3 antibodies, and IgG as negative control. Subsequent qPCR was performed using promoter primers for PD-1, CTLA-4, TIM-3, LAG-3, TIGIT, PD-L1, and galectin-9. Data were normalized to input. ChIP analysis of distribution of H3, H3K9me3, and H3K27me3 at PD-1 (a), CTLA-4 (b), TIM-3 (c), LAG-3 (d), TIGIT (e), PD-L1 (f), and galectin-9 (g) promoters are shown