Fig. 7

Lymph nodes grouped according to NAC response. CD4⁺ T cells from lymph nodes sorted according to the patients’ responses to neoadjuvant chemotherapy (NAC); complete response (CR; pT0N0M0) or no response (NR; pT2 or higher N0M0) Plots show percentage of methylation at signature CpG, evaluated by pyrosequencing, for every lymph node analysed. Lymph node number (n) stated on x-axis beneath sample type. a IFNG (number of patients: CR n = 8, NR n = 3). b IL13 (number of patients: CR n = 4, NR n = 2). c FOXP3 (number of patients: CR n = 6, NR n = 2). d IL17A (number of patients: IL17A CR n = 6 NR n = 2). Mann-Whitney test was used for the statistical analysis. p values are indicated as *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Bars indicate mean with error bars displaying SEM