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Fig. 3 | Clinical Epigenetics

Fig. 3

From: Increased CD4+ T cell lineage commitment determined by CpG methylation correlates with better prognosis in urinary bladder cancer patients

Fig. 3

DNA methylation analysis of CD4+ T cells from PBMC and TILs at time of TUR-B. Methylation levels of a IFNG, b IL13, c FOXP3 and d IL17A signature loci in CD4+ T cells from PBMC and TILs were analysed by pyrosequencing at time of TUR-B. Plots show percentage of methylation for every specimen analysed (n stated on x-axis beneath sample type). p values are indicated as *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001, using Kruskal-Wallis test. Bars indicate mean with error bars displaying SEM. Downward arrow along y-axis illustrate increased lineage commitment, as a result of decreased methylation. e Bar graphs depicting demethylation levels at the four signature sites (Th1 = IFNG, Th2 = IL13, Treg = FOXP3, Th17 = IL17A) from two patients, selected for their low vs high methylation pattern in IFNG. TILs from patient no. 30 with low methylation levels in IFNG (top bars) and patient no. 28 with high methylation in IFNG

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