Fig. 2

CD4⁺ T cell lineage commitment in urinary bladder cancer. CD4⁺ T cells sorted from PBMC, LN and TILs were analysed by pyrosequencing at CD4⁺ T cell signature loci: a IFNG for Th1, b IL13 for Th2, c FOXP3 for Treg and d IL17A for Th17. p values stated in graphs are generated from Kruskal-Wallis test. p values from Dunn’s multiple comparisons test are indicated as *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. Plots show percentage of methylation for every specimen analysed (n stated on x-axis beneath sample type). Bars indicate mean with error bars displaying SEM. Downward arrow along y-axis illustrate increased lineage commitment, as a result of decreased methylation