Fig. 5
PBIT suppresses JARID1B expression and inhibits CSCs-like phenotype. a The molecular structure of PBIT (C14H11NOS), with molecular weight of 241.31 g/mol. b Western blot showed the dose-dependent inhibition of expression of JARID1B, Oct4, Sox2, vimentin, MAPK, STAT3, FAK expression, as well as upregulation of E-cadherin and H3K4me3 by PBIT treatment. β-Actin served as the loading control. c Tumorsphere formation assay showed the effects of JARID1B knockdown and/or 5 μM PBIT treatment in NSCLC-derived tumorspheres. d The histograms of data in c. shJARID1B, JARID1B shRNA-silenced; NC, negative control. The bars were representative of mean ± SEM of independent experiments performed in triplicate assays. *p < 0.05; **p < 0.01; ***p < 0.001