Fig. 3

JARID1B knockdown changes EMT, apoptosis markers and suppresses cell proliferation, colony formation, and migration/invasion of NSCLC cells in vitro. a The knockdown efficiency of two JARID1B shRNAs (JARID1B shRAN-1 and shRNA-2) against endogenous JARID1B were evaluated by Western blot. Accompanied changes of several EMT markers and apoptosis makers were also noted. H3K4me3 increased after JARID1B suppression. ß-Actin served as the loading control. b SRB assay showed JARID1B knockdown suppressed cell proliferation. c (upper panel) JARID1B knockdown suppressed the ability of the H1299 and H441 cells to form colonies. (lower panel) Histograms showed significant inhibition of colony formation in the knockdown clones as compared to the control cells. d Staining of cells in migration assay and invasion assay (left panels) with crystal violet showed significantly reduced migration and invasion, respectively, in H1299 and H441 cells infected with JARID1B shRNA. (right panel) Histograms of the abovementioned data. The bars were representative of mean ± SEM independent experiments performed in triplicate assays. *p < 0.05; **p < 0.01. Original magnification, × 40