Fig. 6
From: Effects of novel HDAC inhibitors on urothelial carcinoma cells
Effects of 19i treatment on gene expression and acetylation status in HDAC4-overexpressing UCCs. Gene expression and protein acetylation in VM-CUB1 and UM-UC-3 cell lines lentivirally transduced to overexpress HDAC4 compared to the parental cells and cell lines transduced with empty vector (LV). a Expression of HDAC4, HDAC5, HDAC7, and HDAC9 mRNAs. The calculated significances refer to the respective parental cell line (*p < 0.05). b TS and p21CIP1 mRNA expression following treatment with 19i (2 μM) or SAHA (2.5 μM) for 48 h. The calculated significances refer to the DMSO solvent control (*p < 0.05). Acetylation of α-tubulin and histones H3 or H4 after 19i treatment (1 and 2 μM) after 48 h. Data in a and b are mean from n = 3; the blot in c shows a representative experiment